algorithms fiji version 1 54 open source (Addgene inc)
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algorithms fiji version 1 54 open source
Algorithms Fiji Version 1 54 Open Source, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+fiji+version+1+54+open+source/MtPT4+(Plasmid+%23117096)/pm41832956-224-238-233
Average 92 stars, based on 3 article reviews
Algorithms Fiji Version 1 54 Open Source, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+fiji+version+1+54+open+source/MtPT4+(Plasmid+%23117096)/pm41832956-224-238-233
Average 92 stars, based on 3 article reviews
algorithms fiji version 1 54 open source - by Bioz Stars,
2026-09
92/100 stars
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Bicinchoninic Acid Protein Assay:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 Luciferase:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 Real-time Polymerase Chain Reaction:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 Negative Control:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 Recombinant:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 Plasmid Preparation:Article Title: Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tetramethylrhodamine ethyl ester Invitrogen Cat#T669; CAS#115532-52-0 MitoSOXTM Red Mitochondrial Superoxide Indicator Invitrogen Cat#M36008 Antimycin A Sigma Cat#A8674; CAS#1397-94-0 CellTrackerTM Deep Red Invitrogen Cat#C34565 Lipofectamine RNAiMAX reagent Invitrogen Cat#13778030 13C palmitic acid Cambridge Isotopes Cat#CLM-409; CAS#56599-85-0 L-carnitine Invitrogen Cat#A17618.09; CAS#541-15-1 L-norvaline Sigma Cat#N7627; CAS#6600-40-4 Methanol Sigma Cat#34860-4L-R; CAS#67-56-1 Chloroform Sigma Cat#C2432; CAS#67-66-3 Methoxamine reagent Thermo Scientific Cat#TS-45950 MtBSTFA +1% t-BDMCS Regis Technologies Cat#1-270144-200 Critical commercial assays L-Lactate Assay Kit Cayman Chemical Cat#700510 Seahorse XF Cell Mito Stress Test Kit Agilent Cat#103015-100 Seahorse XF Glycolysis Stress Test Agilent Cat#103020-100 PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23227 Myco-Sniff-RapidTM Mycoplasma Luciferase Detection Kit MP Biomedicals Cat#0930504-CF QIAprep Spin Miniprep Kit Qiagen Cat#27104 PureLink RNA Mini Kit Invitrogen Cat#12183025 DNeasy Blood & Tissue Kit Qiagen Cat#69504 Experimental models: Cell lines NIH 3T3 murine fibroblasts ATCC Cat#CRL-1658; RRID:CVCL_0594 Immortalized human reduction mammoplasty fibroblasts64 Dr. Charlotte Kuperwasser, Tufts University https://doi.org/10.1073/ PNAS.0401064101 4T1 murine triple negative breast cancer cells ATCC Cat#CRL-2539; RRID:CVCL_0125 HEK293T cells ATCC Cat#CRL-3216; RRID:CVCL_0063 Experimental models: Organisms/strains 8-10-week-old female BALB/c mice Charles River Laboratories RRID:IMSR_APB:4790 Oligonucleotides shScramble in a pLKO.1 backbone: 5′-CCGGCAACAAGATGAAGAGCA CCAACTCGA GTTGGTGCTCTTCAT CTTGTTGTTTTT-3′ Sigma Cat#SHC002 shATG5 in a pLKO.2 backbone: 5′-AGCCGAAGCCTTTGC TCAATG-3′ Sigma Cat#TRCN0000375819 Custom DNA Oligos for qPCR Sigma Cat#VC00021 Silencer Select siRNA targeting Mfn2 (Assay ID s100687) Invitrogen Cat#4390771 Silencer Select Negative Control No. 1 Invitrogen Cat#4390843 |